Stoichiometry and apparent dissociation constant of the calcium-arsenazo III reaction under physiological conditions
نویسندگان
چکیده
منابع مشابه
Spectrophotometric determination of reaction stoichiometry and equilibrium constants of metallochromic indicators. II. The Ca2+-arsenazo III complexes.
The analytical method described in the preceding article was applied to spectrophotometric Ca2+-titrations of the metallochromic indicator arsenazo III (Ar). At various reactant concentrations it was determined that Ar forms 1:1,1:2 and 2 : 1 complexes with calcium. The equilibrium constants and extinction coefficients at 602 nm were determined. Corrected to zero ionic strength at 293 K and pH ...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1980
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(80)85025-9